Hygiyenic Assessment of The Toxicological Propertiyes of The Biological Active Additive “Nutromix”

Abstract

This article incorporates information from India’s «Hexagon Nutrition (Exports) Pvt. Ltd», the results of an experimental assessment of the toxicological safety of the biologically active additive «NUTROMIXTM vitamin & mineral food supplement» for children. The presence of 16 types of vitamins and minerals in the composition of the selected biologically active supplement «NUTROMIX» has been assessed for toxicological safety. Experimental studies were carried out on experimental animals (non-breeding white rats and mice, guinea pigs and rabbits) in accordance with the current normative-methodological base. The biologically active additive «NUTROMIX» has been evaluated for average death value (LD50) when peroral is administered, cumulative effect (subchronic) of biologically active additive «NUTROMIX», mucosal irritating property of biologically active additive, sensitizing effect of biologically active additive. While experimental animals received the same amount of the inspection object corresponding to mg/kg of body weight at the 16-20 hours observation limit, animals in the control group received a suitable amount of distilled water. During the observation, the body weight of rats changed, but it turned out, no sharp difference in body weight changes was detected in the animals in the experimental group and in the animals in the control group. When assessing the toxicity indicators of the product, the animals in the experiment were monitored for 28 days (4 weeks) in accordance with the methodological manual. In the study of the acute toxicity of the biologically active additive «NUTROMIX» in experimental conditions, 2 types of laboratory animals (non-breeding white rats and mice) were administered orally with a gastric tube at the same time using Working Solutions, the amount of the drug in 2000, 4000 and 6000 mg/kg per kilogram of animal weight. The maximum dose of the biologically active additive sent to the stomach by experimental animals for study is calculated 12 times more than the daily physiological need of a person. In the following days, it was observed that the body weight of experimental animals increased, the survival of normal reactions to external influences, their general condition improved. The lack of records of deaths among experimental animals made it impossible to calculate LD50 (average death dose) for a sample of the biologically active additive «NUTROMIX» being studied. A 0.05 ml solution (2 drops) of «NUTROMIX» biologically active additive was dripped into the conjunctival space of the right eye of the guinea pig, while the Left Eye performed a control function. During the observation, no cases of hyperemia, irritation of the mucous membrane or blepharospasm were detected in animals under the experiment. Differences between the experimental and control groups were not detected on the results of macro - and microscopic examination of the studied organs. When all the animals in the experimental group were ruptured, no fluids were detected in the chest and abdomen. No changes were detected in the thyroid gland, the inner intima floor of the aorta, the pericardium, the shape and size of the heart, the left and right ventricles, trachea and large bronchi, the lining of the esophagus and stomach, the inner layers of the small and large intestines, the shape and size of the liver, pancreas, spleen, size and shape of the kidneys, adrenal glands, bladder, biologically active additive «NUTROMIX» - no negative changes were observed in the health cases of laboratory animals in which the experiment was carried out, non–toxic in acute experiments (Class 4 - less toxic), cumulation was not detected, did not have an irritating and sensitizing effect, dystrophic, necrotic and inflammatory processes were not detected in internal limbs.