Immunohistochemical Age-Related Characteristics of Myocardium in Chronic Ischemic Heart Disease

Abstract

In the immunohistochemical examination of the myocardium in chronic ischemic heart disease (CIHD), the following markers were primarily used: Desmin, SA sarcomeric actin, and the myogenic regulatory factor MyoD1. The desmin marker used in this study represents a protein structure forming part of the intermediate filaments of myocardial myocytes and acts as a structural element determining muscle contractility, detectable in both skeletal and cardiac muscles. This intermediate filament protein is primarily expressed in the cytoplasm of cardiomyocytes, where it appears as an intense yellow staining, reflecting homogeneous expression within healthy muscle components. It is characterized by a uniform yellowish staining pattern in the cytoplasm.