DEVELOPMENT OF A DIAGNOSTIC TEST SYSTEM BY IFA METHOD FOR DETERMINATION OF SPECIFIC ANTIBODIES OF THE BRUCELLIOUS CAUSE IN DONORS

Abstract

Based on analysis of comparative data on peculiarities of brucellosis progression, an assertion can be made that in the latest decade brucellosis in people has been less symptomatic which impedes its diagnostics. In such cases laboratory methods of studying this infection are utterly important. This confirms the importance of improving and developing new, more sensitive and specific means of diagnostics, especially those for rapid testing and instrumental account of test results. A diagnostic test system has been developed for detection of specific antibodies to brucellosis pathogens in indirect enzyme immunoassay in the course of study of human biological material, and the conditions for its application have been optimized. The results are presented to demonstrate that the test system is fairly specific and sensitive, which allows proposing it for routine diagnostics of brucellosis as a complementary or alternative test method.